A Simple and Efficient Dye-based Technique for Rapid Screening of Fungi for L-asparaginase Production
نویسندگان
چکیده
Three hundred and sixty four (364) isolates of tropical soil fungi were screened for L-asparaginase production by rapid plate method using modified Czapek-Dox agar containing L-asparagine, and either bromo cresol purple or phenol red dye as an indicator. Results of the study revealed that total one hundred and thirty five (135) isolates showed positive reaction for L-asparaginase production as indicated by the color change in and around the colonies between 48-72 hours of incubation at 28 0 C. A comparative study of the two indicator dyes with varying concentrations showed bromo cresol purple is a better and efficient indicator for L-asparaginase screening than phenol red. Quantitative estimation of L-asparaginase in the selected fungi showed Aspergillus sp. and Fusarium sp. were good candidates for L-asparaginase production. Anil kumar Meghavarnam and Savitha Janakiraman*
منابع مشابه
A Rapid and Efficient Dye Based Plate Assay Technique for Screening of L-Asparaginase Producing Fungal Strains
L-Asparaginase enzyme has received great attention owing to its potential as anticancer drug and food processing agent. L-Asparaginase producing microbes are conventionally screened on phenol red plates containing L-asparagine as the sole nitrogen source for its growth. However, the contrast of the zone in phenol red plates is not very distinct. To overcome this problem, an alternate methodolog...
متن کاملValidation of a Simple and Rapid Method for Assessment of Intracellular Bacterial Asparaginase
L-Asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.In this study, simple and advantageous method has been validated for rapid and precise determination of intracellular L-Asparaginasein bacterial species. A suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...
متن کاملScreening for Type II L-Asparaginases: Lessons from the Genus Halomonas
Among the two types of bacterial L-asparaginases, only type II enzymes have been used inthe treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine.However, current screening media used for the isolation of L-asparaginase-producingmicroorganisms do not discriminate between the two types of L-asparaginase. During anoptimization study conducted to increase L-aspa...
متن کاملImmobilization of Streptomyces gulbargensis in Polyurethane Foam: A Promising Technique for L-asparaginase Production on
In the present study, Streptomyces gulbargensis and its mutant form, S.gulbargensis mu24, immobilized on polyurethane foam were investigated for the production of L-asparaginase using groundnut cake extract as medium. The medium with an initial pH of 8.5 was inoculated with free and immobilized cells separately and then subjected to fermentation by incubation at 40oC and shaking at 200 rev/min....
متن کاملScreening for Type II L-Asparaginases: Lessons from the Genus Halomonas
Among the two types of bacterial L-asparaginases, only type II enzymes have been used inthe treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine.However, current screening media used for the isolation of L-asparaginase-producingmicroorganisms do not discriminate between the two types of L-asparaginase. During anoptimization study conducted to increase L-aspa...
متن کامل